CD45 Polyclonal Antibody

Background:
CD45, also known as leukocyte common antigen (LCA), B220, and T200, is a 180-240 kD single chain type I membrane glycoprotein. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 is one of the best negative selection markers for characterizing and/or isolating human mesenchymal stem cells from bone marrow and other sources. Along with the positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.

Research Area: Cancer, Cardiovascular, Immunology, Neuroscience, Signal Transduction, Stem Cells

Synonyms:
B220, CD 45, CD45, CD45 antigen, CD45R, GP180, L-CA, LCA, Leukocyte common antigen, loc, Ly-5, LY5, Ly5, homolog of, Lyt-4, OTTHUMP00000033813, OTTHUMP00000033816, OTTHUMP00000033817, OTTHUMP00000038574, Protein tyrosine phosphatase receptor type c polypeptide, Protein tyrosine phosphatase, receptor type C, protein tyrosine phosphatase, receptor type, C, Protein tyrosine phosphatase, receptor type, c polypeptide, Ptprc, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200, T200 glycoprotein, T200 leukocyte common antigen

Immunogen: Recombinant protein corresponding to Mouse Human CD45.

Swissprot: P08575,P06800

Calculated MW: 146 kDa
Observed MW: 180-240 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 1% BSA and 50% glycerol, pH7.4

Concentration: 1.15 mg/mL
Dilution: WB 1:500-1:2000

Storage:
Store at -20°C. Avoid freeze / thaw cycles.