CD45R0 (clone EBS-CD-027), anti-human

Source:
A BALB/c mouse was immunized with lentil lectin purified glycoproteins from leucocytes from a B-cell CCL patient.
Fusion partner: NS-1.



Specifications:
CD45 glycoproteins have various molecular weights on various cell types: B-cell 240 kDa, thymocytes 180 kDa, T-cells multiple bands. Reduced in PAGE gels: 180 and 240 kDa. Relevant epitopes are termed CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45R or CD45R0 (exons 4-6 spliced out) EBS-CD-027 reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B-cells or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemia’s. It rarely stains NK-cells or B-cell lymphomas.

Species reactivity:
Positive: human.

Applications:
MAbs against CD45 can be used to differentiate lymphomas from carcinomas. In Hodgkin’s disease CD45 is usually not expressed.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +

Positive control: CCRF-CEM, Jurkat or MOLT-4 cells. Paracortex in tonsil or reactive lymph node.

References:
- Sparrow RL et al,Transplantation36:166 171 (1983)