CD45RB (clone 128-4G2), anti- human

Source:
A BALB/c mouse was immunized with stimulated human leucocytes.
Fusion partner: NS-1.



Specifications:
CD45 glycoproteins have various molecular weights on various cell types: B-cell 240 kDa, thymocytes 180 kDa, T-cells multiple bands. Reduced in PAGE gels: 180 and 240 kDa. Relevant epitopes are termed CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45R or CD45R0 (exons 4-6 spliced out). 128-4G2 reacts with CD45RB, which is expressed on mature B-lymphocytes and the majority of lymphomas and leukemias of B-cell origin. CD45RB is also expressed on cells of myeloid lineage.

Species reactivity:
Positive: human. Negative: rat.

Applications:
MAbs against CD45 can be used to differentiate lymphomas from carcinomas. In Hodgkin’s disease CD45 is usually not expressed.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: Citrate

Positive control: Ramos, U-698, or GA-10 cells. Tonsil.

References:
- Schlosssman S.F. et al. In Leukocyte typing V, Oxford University Press, 501,631, 644, 727 (1995).