CD46 (clone 197-2B1), anti-human

Source:
A BALB/c mouse was immunized with stimulated human leucocytes.
Fusion partner: NS-1.



Specifications:
197-2B1 reacts with human CD46, a 52-58 kDa single chain type 1 transmembrane molecule. CD46 is a cofactor for the factor I regulation of complement components C3b and C4b and contains four N-terminal short consensus repeat (SCR) modules. It is strongly expressed on salivary gland ducts and kidney ducts and often increased on tumor cells. It is also serves as a measles virus receptor, an adherence factor for group A Streptococcus pyogenes, and a cellular pilus receptor for pathogenic Neisseria. 197-2B1 has been clustered at the VIth HLDA Workshop.

Species reactivity:
Positive: human.

Applications:
197-2B1 can be applied to test complement activation in pseudo-allergic reactions to acetylsalicylic acid and test for measles virus infection of cells.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +

Positive control: K562, U937, HL60, HeLa and Jurkat cells, and human lymphocytes. Lymph node and tonsil.

References:
- Kishimoto T. et al., eds. Leukocyte Typing VI, p506-509 and p1145-1146, Garland Publishing, Inc., New York and London, (1997).
- Sandilands, G. et al. Clin. and Exp. Immunol.162(3): 516-27 (2010).