CD47 (clone BRIC 126 ) Affinity Purified, anti-human

Decription:
Mouse monoclonal antibody to human CD47 Affinity purified. CD47 is a heavily N-glycosylated hydrophobic cell membrane glycoprotein of apparent molecular weight 45-60 kDa on erythrocytes and 47-55 kDa on platelets. Analysis of the CD47 amino acid sequence predicts that the protein has an extracellular immunoglobulin (IgV)-like domain at the amino-terminus (about 120 amino acids), five membrane spanning segments (about 152 amino acids), and a short hydrophobic intracytoplasmic tail (about 30 amino acids). The gene encoding human CD47 is located on q.1-.2 on human chromosome . The glycoprotein function is of an adhesion and thrombospondin receptor. CD47 has a very broad tissue distribution and is present on hemopoetic cells, epithelial cells, fibroblasts, brain, tumour cell lines, mesenchymal cells. There are approximately 50,000 CD47 molecules per erythrocyte. The glycoprotein is deficient in erythrocytes of the rare Rh null phenotype.

Synonyms: CD47, CD-47

Clone: BRIC 126

Isotype: Mouse IgG2b kappa light chain

Immunogen: BRIC 126 was made in response to human erythrocytes.

Reactivity: human

Form: Affinity purified, in 0.1 M glycine buffer, 1 M Tris buffer, pH 7.4, sterile filtered

Applications: Immunoblotting, Immunofluorescence

Working Dilution: Immunofluorescence, Immunoblotting. It has a functional binding affinity for erythrocytes of 2.8x10^8 M-1

Storage: 2-8C for immediate use, or at -20C (aliquot)

References:
1. Hadam, MR., (1989) in Leucocyte Typing IV; White Cell Differentiation Antigens, Ed. W. Knapp et al. Oxford University Press pp. 658-660.
2. Avent ND. et al., (1988), Biochem. J. 251: 499-505
3. Gardner B et al. (1991) Transfusion Med. 1, 77-85.
4. Mawby, WJ et al. (1994) Biochem J. 304: 525-530
5. Anstee DJ et al.(1993) Proceedings of the fifth workshop and conference on white cell differentiation antigens, Boston, vol. 2: 233-234
6. Yuan, FF & Fletcher, A (1997) Proceedings of the sixth workshop and conference on white cell differentiation antigens, Japan, 1996, 382-385 eds. Kishimoto et al. Garland publishing, Inc. NY & London