CD49b/CD29 (clone P-16) Affinity Purified, anti-human

Decription:
Mouse monoclonal antibody to human CD49b/CD29-Affinity purified. The CD49b (GpIa) protein is the alpha subunit of the integrin GpIa/IIa heterodimer (CD49b/CD29). Integrins are heterodimeric integral membrane glycoproteins composed of a distinct alpha chain and a common beta chain and are found on a wide variety of cell types including, T cells (the NKT cells), NK cells, fibroblasts and platelets. Integrins are involved in cell adhesion and also participate in cell-surface mediated signalling. On platelets, the GpIa/IIa complex functions primarily as a ligand for collagen.

Synonyms: CD49b/CD29 antigen, GpIa protein, alpha subunit of the integrin GpIa/IIa heterodimer

Clone: P-16

Isotype: Mouse IgG1, kappa light chain

Immunogen: P-16 was made in response to immunisation with Glanzmann s platelets (lacking GpIIb/IIIa).

Reactivity: human

Form: Affinity purified, in 0.1 M glycine buffer, 1 M Tris buffer, pH 7.4, sterile filtered

Applications: Immunoblotting, Flow Cytometry, Immunofluorescence

Working Dilution: Optimal dilution should be performed by user

Storage: 2-8C for immediate use, or at -20C (aliquot)

References: Campbell K, Rishi K, Howkins G, Gilby D, Mushens R, Ghevaert C, Metcalfe P, Ouwehand W, Lucas G (2007). A modified rapid monoclonal antibody-specific immobilisation of platelet antigen assay for the detection of human platelet antigens (HPA) antibodies: a multicentre evaluation. Vox Sanguinis, 93, 289-297