CD49f (Alpha 6 Integrin) (clone GoH3), anti-human

Background:
The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Two transcript variants encoding different isoforms have been found for this gene.

Decription:
In cutaneous melanocytic lesions, the level of expression of three Integrin subunits (alpha 2, alpha 6, beta 3) has been found to be correlated with tumor progression.

Synonyms: CD49f, VLA-6, ITGA6B

Clone: GoH3

Isotype: Rat IgG2a

Immunogen: Mouse mammary tumor cells

Reactivity: human

Specificity: Mab GoH3, directed against alpha6 Integrins (Laminin receptor, VLA-6) shows a cytoplasmic staining pattern of uveal and cutaneous melanoma cells

Form: Hybridoma culture supernatant

Applications: Immunoblotting, Flow Cytometry, Immunohistochemistry, suitable for frozen sections

Working Dilution: 1:5 to 1:10

Reactivity: Epithelial tissues

Storage: 2-8C for immediate use; or at -20C (aliquot). Avoid freeze-thaw cycles.

References:
ten Berge, PJM et al. (1993) Investigative Ophthalmology & Visual Science Vol 34, No. 13.
Sonnenberg A et al. (1987) J Biol Chem 262: 10376-10383
Bosman FT et al. (1993) Int J Dev Biol 37: 203-211
Bosnan FT. et al. (1993) Histochem J 25: 469-477