CD50 (ICAM-3) (clone SPM505), anti-human
€0.00
In stock
SKU
ARP-24-3385-MSM1X
Catalog Number: 24-3385-MSM1X
Size: 100 µl, 0.5 ml, 1 ml
Isotype: Mouse IgG2b kappa
Size: 100 µl, 0.5 ml, 1 ml
Isotype: Mouse IgG2b kappa
Decription:
Recognizes the D1 domain of an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This monoclonal antibody is excellent for staining of formalin/paraffin tissues.
Synonyms: ICAMR, Intercellular adhesion molecule 3 (ICAM3)
Clone: SPM505
Isotype: Mouse IgG2b kappa
Immunogen: Stimulated human leukocytes
Reactivity: human. Others not known.
Form: Purified, in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Applications: Blocks binding of CD11a (LFA-1) to CD50 (ICAM-3) (Order Ab without Azide)
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Reactivity: HL-60 or THP-1 cells. Lymph node and tonsil
Concentration: 0.2mg/ml
Storage: Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
Recognizes the D1 domain of an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This monoclonal antibody is excellent for staining of formalin/paraffin tissues.
Synonyms: ICAMR, Intercellular adhesion molecule 3 (ICAM3)
Clone: SPM505
Isotype: Mouse IgG2b kappa
Immunogen: Stimulated human leukocytes
Reactivity: human. Others not known.
Form: Purified, in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Applications: Blocks binding of CD11a (LFA-1) to CD50 (ICAM-3) (Order Ab without Azide)
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Reactivity: HL-60 or THP-1 cells. Lymph node and tonsil
Concentration: 0.2mg/ml
Storage: Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
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