CD58 (LFA-3) (clone BRIC 5) Affinity Purified, anti-human

Decription:
Mouse monoclonal antibody to human CD58 (LFA-3) Affinity purified. CD58, or LFA3, is a membrane glycoprotein of 55kDa to 70kDa and is mapped to chromosome 1p. It occurs in two forms, one transmembrane with a cytoplasmic domain, the other form anchored in the membrane via a glycosylphosphatidylinositol tail. The complete amino acid sequence of both forms has been deduced from cDNA. It is heavily N-glycosylated. CD58 is a cell adhesion molecule which plays a critical role in facilitation of antigen specific recognition through interaction with CD2 on T lymphocytes and natural killer cells. It is a member of the immunoglobulin superfamily of molecules. CD58 has a wide tissue distribution, being present on erythrocytes, platelets, monocytes, a subset of lymphocytes, bone marrow cells, fibroblasts, epithelium and endothelium cells. There are approximately 5000 CD58 molecules on each erythrocyte. There is reduced expression of CD58 on cells of individuals with paroxysmal nocturnal haemoglobinuria.

Synonyms: CD58, LFA3, Cell adhesion molecule, Ag3, Surface glycoprotein LFA-3

Clone: BRIC 5

Isotype: Mouse IgG2a kappa light chain

Immunogen: BRIC 5 was made in response to human erythro­cytes. It inhibits human T cell rosette formation and blocks T cell proliferation stimulated by activated T cells.

Reactivity: human

Form: Affinity purified, in 0.1 M glycine buffer, 1 M Tris buffer, pH 7.4, sterile filtered

Applications: It reacts by Immunoblotting to non-reduced erythrocyte membranes.

Working Dilution: The functional affinity of BRIC 5 binding to erythrocytes is 4 x 10^8 M-1

Storage: 2-8C for immediate use, or at -20C (aliquot)

References:
1. Makgoba M.W., et al, (1989) Immunology Today 10 417-422 (Review)
2. Shaw S. & Johnson J.P., (1989) in Leucocyte Typing IV; White Cell Differentiation Antigens Ed. W. Knapp et al Oxford University Press pp 714-716
3. Klickstein et al., (1993) Proceedings of the fifth workshop and conference on white cell differentiation antigens, Boston, vol. 2 1475-1476.
4. Anstee DJ et al. (1991) Immunology, 74: 197-205.
5. Schlossman SF (ed) et al., (1993) Proceedings of the fifth workshop and conference on white cell differentiation antigens, Boston, Oxford University Press 1995.