CD59 (clone BRIC 229) Affinity Purified, anti-human

Decription:
Mouse monoclonal antibody to human CD59 Affinity purified. CD59 (also known as HRF20, MIRL, P18, H19, MAC-inhibitor) is a cell surface glycoprotein of apparent molecular weight 18-20 kDa which contains N- glycans. It is attached to the cell surface by a glycosylphosphatidylinositol tail. The full amino acid sequence derived from cDNA is known. CD59 has been mapped to chromosome 11p14-. It is a complement regulatory protein. It inhibits the terminal stage of the formation of membrane attack complexes by homologous complement activation. CD59 is broadly distributed among haemopoietic and non - haemopoietic cells such as B cells, T cells, monocytes, epithelium, platelets, polymorphonuclear neutrophils and endothelium. Daudi and U937 cells are unreactive. There are approximately 20000-40000 CD59 molecules per erythrocyte. There is reduced expression of CD59 on cells of individuals with paroxysmal nocturnal haemoglobinuria (PNH).

Synonyms: CD59, HRF20, MIRL, P18, H19, MAC-inhibitor

Clone: BRIC 229

Isotype: Mouse IgG2b kappa light chain

Immunogen: BRIC 229 was made in response to human erythrocytes. The antigen on erythrocytes is sensitive to treatment with Pronase or 6% aminoethylisothiouronium bromide.

Reactivity: human

Form: Affinity purified, in 0.1 M glycine buffer, 1 M Tris buffer, pH 7.4, sterile filtered

Applications: Immunoblotting, Neutralization

Working Dilution: ELISA, Immunoblotting. It has a functional binding affinity for erythrocytes of 2.8x10^8 M-1

Storage: 2-8C for immediate use, or at -20C (aliquot)

References:
1. Ojcius et al (1990) Immunology Today 11, 47-49 (Review).
2. Hadam M.R. (1989) in Leucocyte Typing IV; White Cell Differentiation Antigens Ed. W. Knapp et al Oxford University Press pp 720-722.
3. Fletcher A et al. (1992) Immunology, 75: 507-512.
4. van den Berg CW et al.( 1994) J. Immunol., 4095-4101.
5. Zaltzman et al., (1995) Biochem. J., 307: 651-656.
6. Klickstein et al., (1993) Proceedings of the fifth workshop and conference on white cell differentiation antigens, Boston , vol. 2 p1476-1477.