CD62E polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
E-Selectin(SELE), also called Selectin E, ELAM1 or ELAM, is a cell adhesion molecule expressed only on endothelial cells activated by cytokines. It is though to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. E-Selection is a member of the selectin family of cell adhesion molecules. The E-Selectin gene is mapped on 1q24.2. The ELAM gene is present in single copy in the human genome and contains 14 exons spanning about 13 kb of DNA by Collins et al. Using affinity chromatography, pull-down assays, and mass spectrometric analysis on adenocarcinoma cell lines, Gout et al. showed that SELE bound death receptor-3 on the cancer cells. Western blot analysis revealed that the SELE-binding protein was recognized by anti-DR3 antibodies. Anti-DR3 or knockdown of DR3 by small interfering RNA decreased adhesion of colon cancer cells to SELE and to SELE-expressing endothelial cells.

Description:
Rabbit IgG polyclonal antibody for E-selectin (SELE) detection. Tested with WB, IHC-P in Human, Mouse, Rat.

Synonyms:
CD 62E antibody, CD26E antibody, CD62 antigen-like family member E antibody, CD62E antibody, CD62E antigen antibody, E selectin antibody, E-selectin antibody, ELAM 1 antibody, ELAM antibody, ELAM-1 antibody, ELAM1 antibody, Endothelial adhesion molecule 1 antibody, Endothelial leukocyte adhesion molecule 1 antibody, ESEL antibody, LECAM 2 antibody, LECAM2 antibody, Leukocyte endothelial cell adhesion molecule 2 antibody, Leukocyte-endothelial cell adhesion molecule 2 antibody, LYAM2_HUMAN antibody, SELE antibody, Selectin E antibody, SelectinE antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the N-terminus of human CD62E(33-50aa YDEASAYCQQRYTHLVAI), different from the related rat and mouse sequences by two amino acids.A synthetic peptide corresponding to a sequence at the N-terminus of human CD62E(33-50aa YDEASAYCQQRYTHLVAI), different from the related rat and mouse sequences by two amino acids.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, rat .
Predicted to work with: mouse.

Applications: WB, IHC-P

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.