CD66b (granulocytes) (clone BIRMA 17C) Affinity Purified, anti-human

Decription:
Mouse monoclonal antibody to human CD66b (granulocytes)-Affinity purified. The CD66b antigen, a member of the immunoglobulin (Ig) superfamily, is a 95-100 kDa membrane bound protein encoded by a gene located on chromosome 19. The molecular structure of CD66b reveals a leader sequence of 34 amino acids (aa), an extracellular region of 284 aa and a 29aa hydrophobic domain replaced by GPI. CD66b has 2 Ig C2 domains and 1 IgG-V like domain. The CD66b antigen is expressed on granulocytes. CD66b exhibits only heterotypic adhesion with CD66c with no homophilic activity, suggesting the possibility that CD66b plays a role in the interaction between granulocytes or between granulocytes and epithelial cells.

Synonyms: CD66b antigen, CD66b, CD-66b

Clone: BIRMA 17C

Isotype: Mouse IgG1, kappa light chain

Immunogen: BIRMA 17C was produced from a mouse hybridoma derived from the fusion of Balb/c spleen cells with X63Ag8.653 myeloma cells.

Reactivity: human

Form: Affinity purified, in 0.1 M glycine buffer, 1 M Tris buffer, pH 7.4, sterile filtered

Applications: Immunoblotting, Flow Cytometry, Immunofluorescence

Working Dilution: Optimal dilution should be performed by user

Storage: 2-8C for immediate use, or at -20C (aliquot)

References:
1. Skubitz KM. et al, (Review) PROW CD66b (1999).
2. Skubitz KM. et al, (1997) Leucocyte Typing VI CD66 family workshop panel report p992-1000: sixth international workshop and conference on human leucocyte differentiation antigens, Japan, 1996 Garland Publishing Inc.