CD68 (Macrophage Marker) (clone KP1 + C68/684), anti-human, monkey and cat

CD68 (Macrophage Marker) (clone KP1 + C68/684), anti-human, monkey and cat

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In stock
SKU
ARP-24-968-MSM3
Catalog Number: 24-968-MSM3
Size: 100 µl, 0.5 ml, 1 ml
Isotype: Mouse IgG's
Decription:
This antibody recognizes a glycoprotein of 110kDa, which is identified as CD68. It is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.

Synonyms: GP110, LAMP4, Microsialin, Macrosialin, SCARD1, Scavenger Receptor Class D Member-1

Clone: KP1 + C68/684

Isotype: Mouse IgG's

Immunogen: Subcellular fraction of human alveolar macrophages (KP1); Recombinant human CD68 protein (C68/684)

Reactivity: human, monkey and cat. Others not known.

Form: Purified, in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Applications: Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Immunohistology (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Reactivity: Tonsil, Lymph Node or Spleen

Concentration: 0.2mg/ml

Storage: Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
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