CD95 (FAS, TNFRSF6) (clone B-R18), anti-human
€0.00
In stock
SKU
ARP-24-355-MSM1
Catalog Number: 24-355-MSM1
Size: 100 µl, 0.5 ml, 1 ml
Isotype: Mouse IgG1, kappa
Size: 100 µl, 0.5 ml, 1 ml
Isotype: Mouse IgG1, kappa
Decription:
monoclonal antibody B-R18 specifically recognizes CD95, also known as Fas, a transmembrane glycoprotein with a MW of 40-45kDa, containing 8kDa of N-glycosidic-linked polysaccharide. It is a receptor for TNFSF6/FASLG, a member of the nerve growth factor receptor/tumor necrosis factor superfamily, mediating receptor-triggered apoptosis. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation, which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). CD95 antigen is expressed on the surface of various cell types, preferentially on the CD45RAlow CD45ROhigh subset of memory T lymphocytes.
Synonyms: ALPS1A, Apoptosis APO1 antigen 1, Apoptosis-mediating surface antigen FAS, APT1, Delta Fas/APO1/CD95, Fas (TNF receptor superfamily, member 6), Fas AMA, Fas cell surface death receptor, FAS1, FASL receptor, FASLG receptor, FASTM, Surface antigen APO1, TNF receptor superfamily member 6 (TNFR6), Tumor necrosis factor receptor superfamily member 6 (TNFRSF6)
Clone: B-R18
Isotype: Mouse IgG1, kappa
Immunogen: Recombinant human CD95 protein
Reactivity: human. Others not-tested.
Form: Purified, in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Applications: Functional Studies (Order Ab without Azide)
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Immunohistology (Frozen) (0.5-1ug/ml for 30 minutes at RT)
Optimal dilution for a specific application should be determined.
Reactivity: MCF-7 cells; hepatocellular or bladder carcinoma
Concentration: 0.2mg/ml
Storage: Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
monoclonal antibody B-R18 specifically recognizes CD95, also known as Fas, a transmembrane glycoprotein with a MW of 40-45kDa, containing 8kDa of N-glycosidic-linked polysaccharide. It is a receptor for TNFSF6/FASLG, a member of the nerve growth factor receptor/tumor necrosis factor superfamily, mediating receptor-triggered apoptosis. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation, which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). CD95 antigen is expressed on the surface of various cell types, preferentially on the CD45RAlow CD45ROhigh subset of memory T lymphocytes.
Synonyms: ALPS1A, Apoptosis APO1 antigen 1, Apoptosis-mediating surface antigen FAS, APT1, Delta Fas/APO1/CD95, Fas (TNF receptor superfamily, member 6), Fas AMA, Fas cell surface death receptor, FAS1, FASL receptor, FASLG receptor, FASTM, Surface antigen APO1, TNF receptor superfamily member 6 (TNFR6), Tumor necrosis factor receptor superfamily member 6 (TNFRSF6)
Clone: B-R18
Isotype: Mouse IgG1, kappa
Immunogen: Recombinant human CD95 protein
Reactivity: human. Others not-tested.
Form: Purified, in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Applications: Functional Studies (Order Ab without Azide)
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Immunohistology (Frozen) (0.5-1ug/ml for 30 minutes at RT)
Optimal dilution for a specific application should be determined.
Reactivity: MCF-7 cells; hepatocellular or bladder carcinoma
Concentration: 0.2mg/ml
Storage: Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
| Is Featured? | No |
|---|
Write Your Own Review