Cdc25A polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
The human CDC25 tyrosine phosphatases trigger activation of CDC2 by removing inhibitory phosphate from tyrosine and threonine residues of the cyclin-dependent kinases. The genes encoding these phosphatases are suspected of being potential oncogenes because of their role in promoting cell division. Three human CDC25 genes have been identified: CDC25A, CDC25B, and CDC25C. The CDC25A gene is mapped to 3p21. In rodent cells, human CDC25A or CDC25B but not CDC25C phosphatases cooperate with either the gly12-to-val mutation of the HRAS gene or loss of RB1 in oncogenic focus formation.

Description:
Rabbit IgG polyclonal antibody for M-phase inducer phosphatase 1 (CDC25A) detection. Tested with WB, IHC-P in Human, Mouse, Rat.

Synonyms:
Cdc 25a antibody, CDC25A antibody, CDC25A1 antibody, CDC25A2 antibody, CDC25A2 CAG isoform antibody, Cell division cycle 25 homolog A(S. pombe) antibody, Cell division cycle 25A antibody, Cell division cycle 25A isoform a antibody, Cell division cycle 25A isoform b antibody, D9Ertd393e antibody, Dual specificity phosphatase Cdc25A antibody, M phase inducer phosphatase 1 antibody, M-phase inducer phosphatase 1 antibody, MGC115549 antibody, MPIP1_HUMAN antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the N-terminus of human Cdc25A(1-17aa MELGPEPPHRRRLLFAC), identical to the related rat and mouse sequences.A synthetic peptide corresponding to a sequence at the N-terminus of human Cdc25A(1-17aa MELGPEPPHRRRLLFAC), identical to the related rat and mouse sequences.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, rat .
Predicted to work with: mouse.

Applications: WB, IHC-P

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.