Cdc25B polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
Central to the onset of mitosis in all eukaryotic cells is the CDC2 protein kinase, the activity of which is negatively regulated by phosphorylation and positively activated by dephosphorylation. The latter function is carried out by a specific phosphatase, CDC25. At least 3 human CDC25 genes code for the A, B, and C forms of CDC25. CDC25B is mapped to 20p13. P38 kinase has a critical role in the initiation of a G2 delay after ultraviolet radiation. Inhibition of p38 blocks the rapid initiation of this checkpoint in both human and murine cells after ultraviolet radiation. In vitro, p38 binds and phosphorylates CDC25B at serines 309 and 361, and CDC25C at serine-216; phosphorylation of these residues is required for binding to 14-3-3 proteins. In vivo, inhibition of p38 prevents both phosphorylation of CDC25B at serine-309 and 14-3-3 binding after ultraviolet radiation, and mutation of this site is sufficient to inhibit the checkpoint initiation. Regulation of CDC25B phosphorylation by p38 is a critical event for initiating the G2/M checkpoint after ultraviolet radiation.

Description:
Rabbit IgG polyclonal antibody for M-phase inducer phosphatase 2 (CDC25B) detection. Tested with WB, IHC-P in Human, Mouse, Rat.

Synonyms:
AI604853 antibody, Cdc 25B antibody, Cdc25b antibody, CDC25B1 antibody, CDC25B2 antibody, CDC25B3 antibody, CDC25HU2 antibody, Cdc25m2 antibody, Cell division cycle 25 homolog B antibody, Cell division cycle 25B antibody, Cell division cycle 25B isoform 1 antibody, Cell division cycle 25B isoform 2 antibody, Cell division cycle 25B isoform 3 antibody, Cell division cycle 25B isoform 4 antibody, Dual specificity phosphatase Cdc25B antibody, EC 3.1.3.48 antibody, M phase inducer phosphatase 2 antibody, M-phase inducer phosphatase 2 antibody, MPIP2_HUMAN antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
E.coli-derived human Cdc25B recombinant protein (Position: M119-L248). Human Cdc25B shares 71% and 68.2% amino acid (aa) sequence identity with mouse and rat Cdc25B, respectively.E.coli-derived human Cdc25B recombinant protein (Position: M119-L248). Human Cdc25B shares 71% and 68.2% amino acid (aa) sequence identity with mouse and rat Cdc25B, respectively.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: mouse, rat.
Predicted to work with: human.

Applications: WB, IHC-P

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.