Cdc25C polyclonal, anti-human, mouse, rat

Background:
Cell cycle events are regulated by the sequential activation and deactivation of cyclin-dependent kinases (Cdks), including Cdk2 and Cdc2. Cdk2 in complexes with cyclin E and cyclin A appears necessary for the onset and progression of DNA replication, while the Cdc2 kinase in complexes with cyclin A or cyclin B is required for the initiation of cell division. Wee 1 has been identified as a protein kinase that suppresses the entry into mitosis by mediating inhibiting tyrosine phosphorylation of Cdc2 p34. In contrast, members of the Cdc25 family of protein phosphatases function as mitotic activators by dephosphorylation of Cdc2 p34 on regulatory tyrosine and possibly threonine residues. The Cdc25 gene family consists of at least three members that share approximately 40% identity in their most conserved carboxy-terminal sequences.

Alternative Name:
CDC25C, CDC 25, Cdc 25C, CDC25, CDC25C, Cell division cycle 25 homolog C, Cell division cycle 25C, Cell division cycle 25C protein, Dual specificity phosphatase Cdc25C, M phase inducer phosphatase 3, M-phase inducer phosphatase 3, Mitosis inducer CDC25, MPIP3, MPIP3, Phosphotyrosine phosphatase, PPP1R60, protein phosphatase 1, regulatory subunit 60,

Application Dilution:
WB:1:500-1:1,000
ICC:1:500-1:2,000
IHC:1:50-1:200
FC:1:50-1:100

Specificity: Cdc25C polyclonal antibody detects endogenous levels of Cdc25C protein.

Immunogen:
Peptide

MW: 53 kDa

Swiss-Prot: P30307(Human) P48967(Mouse) Unigene:162298(Rat)

Purification & Purity:
Peptide affinity purified.

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.