Cdc27 polyclonal, anti-human, mouse, rat

Background:
n the cell cycle. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with Hsp90. Cdc34, Cdc27 and Cdc16 function as ubiquitin-conjugating enzymes. Cdc34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae Cdc34, which is essential for the G1 to S phase transition. Cdc16 and Cdc27 are components of the APC (anaphase-promoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation.

Alternative Name:
APC3, CDC27Hs, CDC27, ANAPC3, Anaphase Promoting Complex 3, Anaphase promoting complex protein 3, Anaphase Promoting Complex Subunit 3, Anaphase-promoting complex subunit 3, APC 3, APC3, Cdc 27, Cdc27, CDC27 homolog, CDC27, CDC27Hs, Cell Division Cycle 27, Cell division cycle protein 27 homolog, D0S1430E, D17S978E, H NUC, H-NUC, HNUC, Nuc2 homolog,

Application Dilution:
WB:1:1,000-1:5,000

Specificity: Cdc27 polyclonal antibody detects endogenous levels of Cdc27 protein.

Immunogen:
recombinant protein

MW: 92 kDa

Swiss-Prot: P30260(Human) A2A6Q5(Mouse) Q4V8A2(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.