CDC6 (clone DCS-180), anti-human

Background:
In yeasts, Cdc6(Saccharomyces cerevisiae) and Cdc18(Schizosaccharomyces pombe) associate with the origin recognition complex(ORC) proteins to render cells competent for DNA replication. Cdc6 is overexpressed in human cancers, where it has a critical regulatory role in addition to DNA replication. Yan et al.(1998) showed that Cdc6 is expressed selectively in proliferating but not quiescent mammalian cells, both in culture and within tissues in intact animals. During the transition from a growth-arrested to a proliferative state, transcription of mammalian Cdc6 is regulated by E2F proteins, as revealed by a functional analysis of the human Cdc6 promoter and by the ability of exogenously expressed E2F proteins to stimulate the endogenous Cdc6 gene. They conclude that expression of human Cdc6 is regulated in response to mitogenic signals though transcriptional control mechanisms involving E2F proteins, and that Cdc6 is required for initiation of DNA replication in mammalian cells.

Decription:
Mouse IgG monoclonal antibody for CDC6, cell division cycle 6 homolog (S. cerevisiae) (CDC6) detection. Tested with WB in Human. No cross reactivity with other proteins.

Synonyms: CDC18L, HsCdc18, p62(cdc6), CELL DIVISION CYCLE 18, S. POMBE, HOMOLOG-LIKE, CDC6(cell division cycle 6, S. cerevisiae) homolog, CELL CYCLE CONTROLLER CDC6, CDC6-related protein, Cdc18-related protein

Clone: DCS-180

Isotype: Mouse IgG1

Immunogen: Recombinant human Cdc6.

Reactivity: human

Form: Lyophilized; Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Applications: WB

Reconstitution: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.