Cdc6 (Phospho-S54) polyclonal, anti-human

Background:
Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division control (Cdc) genes are required at various points in the cell cycle. Cdc25A, Cdc25B and Cdc25C protein Tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory Tyrosine residues. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with HSP 90. Cdc34, Cdc27 and Cdc16 function as ubiquitin-conjugating enzymes. Cdc34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae Cdc34, which is essential for the G1 to S phase transition. Cdc16 and Cdc27 are components of the APC (x complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation.

Alternative Name:
HsCdc18, HsCDC6, CDC6, Cdc 18L, Cdc 6, CDC18 (cell division cycle 18, S.pombe, homolog) like, CDC18 (S.pombe), Cdc18 related protein, CDC18(S.pombe), Cdc18-related protein, Cdc18L, cdc6, CDC6 cell division cycle 6 homolog, CDC6 related protein, CDC6-related protein, CDC6, Cdc6p, CELL CYCLE CONTROLLER CDC6, Cell division control protein 6, Cell division control protein 6 homolog, Cell division cycle 6 homolog (S. cerevisiae), Cell division cycle 6 homolog, Cell division cycle 6, S. cerevisiae, homolog of, HsCDC 6, HsCDC18, HsCDC6, p62, p62(cdc 6), p62(cdc6),

Application Dilution:
WB:1:1,000-1:2,000
ICC:1:50-1:200

Specificity: Cdc6 (Phospho-S54) polyclonal antibody detects endogenous levels of Cdc6 protein only when phosphorylated at S54.

Immunogen:
Synthetic phospho-peptide corresponding to residues surrounding Ser54 of human Cdc6.

MW: 63 kDa

Swiss-Prot: Q99741(Human)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.