CDK4 monoclonal, anti-human, mouse, rat

CDK4 monoclonal, anti-human, mouse, rat

€455.00
In stock
SKU
AC-ABO13458
Catalog Number: AC-ABO13458
Size: 100 µl
Isotype: Rabbit IgG
Applications: WB, IF, ICC, IP
Datasheet
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Description: Anti-CDK4 Rabbit Monoclonal Antibody . Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Protein Function:
Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G (1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G (1) phase. Hypophosphorylates RB1 in early G (1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex..

Other Names: Cyclin-dependent kinase 4, 2.7.11.22, Cell division protein kinase 4, PSK-J3, CDK4

Clone Name(s):
Clone: HOB-3

Subcellular Localization: Cytoplasm. Nucleus. Membrane. Cytoplasmic when non-complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G (1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G (1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus.

Gene ID: 1019

Immunogen: A synthesized peptide derived from human CDK4

Calculated MW: 33730

Purification: Affinity-chromatography

Format: Liquid

Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Concentration (mg/ml): Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
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