CGRP (Calcitonin gene-related peptide) polyclonal, anti-human, rat

Quantity: 50 µl

Background:
This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.

Synonyms:
CALCA, CALC1, CGRP, CGRP-I, CGRP1, CT, KC

Isotype: Rabbit Polyclonal

Immunogen:
Calcitonin gene-related peptide (synthetic, rat), conjugated to BSA

Form: Serum containing 0.1% sodium azide

Specificity:
Absorption with 10-100 ?g CGRP per ml diluted antiserum abolishes the staining while calcitonin, substance P and Neurokinin A do not.CGRP (Calcitonin gene-related peptide)

Reactivity: human, rat

Applications: Immunofluorescence, Immunohistochemistry on paraffin and frozen sections

Working DIlution: 1:100 to 1:200 using PAP; 1:80 to 1:150 immunofluorescence microscopy with FITC

Positive Control:
Frozen sections of rat colon

Storage: 2-8C for immediate use, or at -20C (aliquot)

References:
- Grundlitz, T. et al. (1986) Endocrinology 119: 2313-2324
- Suzuki, N. et al. (1989) Neuroscience 30: 595-604
- Luts, L et al. (1994) Regulatory Peptides 50: 147-158