Chicken a1AGP (Alpha-1-Acid Glycoprotein) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-1-Acid Glycoprotein(a1AGP). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Alpha-1-Acid Glycoprotein(a1AGP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-1-Acid Glycoprotein(a1AGP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-1-Acid Glycoprotein(a1AGP) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Infection immunity, Hepatology, Gastroenterology, Immunodeficiency

Alternative Names:
alpha1-AGP，AGP1, a1-AGP, ORM1, OMD 1, Orosomucoid 1

Sample Type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Type: Sandwich

Standard: 400 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only