Chicken Leptin (LEP) Detection Kit

Use: Used to detect the concentration of chicken leptin (LEP) in samples such as serum, plasma, and cell culture supernatant.Specificity: This kit uses a double antibody sandwich enzyme-linked immunosorbent assay (ELISA). In a microwell ELISA plate pre-coated with anti-chicken leptin (LEP) antibody (solid phase antibody), chicken leptin (LEP) calibrator and the sample to be tested are added, and then biotin-labeled antibody is added. After incubation and sufficient washing, HRP-coupled avidin is added. After incubation and sufficient washing, unbound components are removed, and a sandwich complex of solid phase antibody-antigen-biotin-labeled antibody-avidin enzyme is formed on the solid surface of the microwell plate. Add TMB colorimetric solution to produce a blue product, which is finally converted into yellow under the action of the stop solution. The absorbance (OD value) is measured at a wavelength of 450nm on the microplate reader. The absorbance (OD value) is positively correlated with the concentration of chicken leptin (LEP) in the sample to be tested. The concentration of chicken leptin (LEP) in the sample can be calculated by fitting the calibrator curve.

Storage Conditions
Store at 2-8°C.

Use: The kit is for research use only.