Chk1 (Phospho-S296) polyclonal, anti-human

Background:
Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2 DNA damage checkpoint by phosphorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee1 in vitro, providing evidence that the hyperphosphorylated form of Wee1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1.

Alternative Name:
CHEK1, C85740, Cell cycle checkpoint kinase, Checkpoint , S. pombe, homolog of, 1, Checkpoint kinase 1, Checkpoint kinase 1 homolog (S. pombe), CHEK 1, Chek1, Chk 1, Chk1, CHK1 checkpoint homolog (S. pombe), CHK1, EC 2.7.11.1, rad27, Serine/threonine protein kinase Chk1, Serine/threonine-protein kinase CHK1, STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae),

Application Dilution:
WB:1:1,000
IHC:1:50-1:200

Specificity: Chk1 (Phospho-S296) polyclonal antibody detects endogenous levels of Chk1 protein only when phosphorylated at S296.

Immunogen:
Synthetic phospho-peptide corresponding to residues surrounding Ser296 of human Chk1

MW: 54 kDa

Swiss-Prot: O14757(Human)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.