CHK1 polyclonal (C-term), anti-human, mouse
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In stock
SKU
AC-AP7401a
Catalog Number: AC-AP7401a
Size: 80 µl, 400 µl
Isotype: Rabbit IgG
Clone Name: RB2529
Applications: WB
Datasheet
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Size: 80 µl, 400 µl
Isotype: Rabbit IgG
Clone Name: RB2529
Applications: WB
Datasheet
Request Information
Background:
Checkpoint pathways control the order and timing of cell cycle transitions and ensure that critical events, such as DNA replication and chromosome segregation, are completed with high fidelity. Antibodies against CHK1 recognize a 54-kD protein on immunoblots of mammalian cell extracts, but the protein has altered mobility when isolated from cells treated with ionizing radiation (IR), indicating that CHK1 is modified in response to DNA damage. In vitro, CHK1 directly phosphorylates a regulator of CDC2 tyrosine phosphorylation, CDC25C. Evidence indicates that in response to DNA damage, CHK1 phosphorylates and inhibits CDC25C, thus preventing activation of the CDC2-cyclin B complex and mitotic entry. Proteolysis of activated CHK1 may promote checkpoint termination under normal conditions and may play a role in the cytotoxic effects of camptothecin and related anticancer drugs.
Other Names:
Serine/threonine-protein kinase Chk1, CHK1 checkpoint homolog, Cell cycle checkpoint kinase, Checkpoint kinase-1, CHEK1, CHK1
Target/Specificity:
This CHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 425-455 amino acids from the C-terminal region of human CHK1.
Antigen Region:
425-455
Antigen Type: Synthetic Peptide
Gene Name: CHEK1
Gene ID: 1111
Primary Accession: O14757
NCBI Accession: NP_001107593.1;NP_001107594.1;NP_001231775.1;NP_001265.2
Other Accession: Q6DE87; Q91ZN7; O35280; Q8AYC9
Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Checkpoint pathways control the order and timing of cell cycle transitions and ensure that critical events, such as DNA replication and chromosome segregation, are completed with high fidelity. Antibodies against CHK1 recognize a 54-kD protein on immunoblots of mammalian cell extracts, but the protein has altered mobility when isolated from cells treated with ionizing radiation (IR), indicating that CHK1 is modified in response to DNA damage. In vitro, CHK1 directly phosphorylates a regulator of CDC2 tyrosine phosphorylation, CDC25C. Evidence indicates that in response to DNA damage, CHK1 phosphorylates and inhibits CDC25C, thus preventing activation of the CDC2-cyclin B complex and mitotic entry. Proteolysis of activated CHK1 may promote checkpoint termination under normal conditions and may play a role in the cytotoxic effects of camptothecin and related anticancer drugs.
Other Names:
Serine/threonine-protein kinase Chk1, CHK1 checkpoint homolog, Cell cycle checkpoint kinase, Checkpoint kinase-1, CHEK1, CHK1
Target/Specificity:
This CHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 425-455 amino acids from the C-terminal region of human CHK1.
Antigen Region:
425-455
Antigen Type: Synthetic Peptide
Gene Name: CHEK1
Gene ID: 1111
Primary Accession: O14757
NCBI Accession: NP_001107593.1;NP_001107594.1;NP_001231775.1;NP_001265.2
Other Accession: Q6DE87; Q91ZN7; O35280; Q8AYC9
Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
| Is Featured? | No |
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