CIAS1/NALP3 polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
NLRP3(NLR FAMILY, PYRIN DOMAIN-CONTAINING 3), also known as CIAS1, CRYOPYRIN, NALP3 or PYPAF1, is a protein that in humans is encoded by the NLRP3(NOD-like receptor family, pryin domain containing 3) gene. The NLRP3 gene encodes a pyrin-like protein expressed predominantly in peripheral blood leukocytes. And the NLRP3 gene is mapped on 1q44. NLRP3 interacts with apoptosis-associated speck-like protein containing a CARD(ASC). The encoded protein may play a role in the regulation of inflammation and apoptosis. Mutation of the NALP3 nucleotide-binding domain reduced ATP binding, CASP1 activation, IL1B production, cell death, macromolecular complex formation, self-association, and association with ASC. Consistent with an essential role for Nlrp3 inflammasomes in antifungal immunity, Gross et al.showed that Nlrp3-deficient mice are hypersusceptible to C. albicans infection. Activation of the NLRP3 inflammasome in response to virus or to RNA was dependent upon lysosomal maturation and reactive oxygen species production in human cells. The NLRP3 inflammasome senses obesity-associated danger signals and contributes to obesity-induced inflammation and insulin resistance.

Description:
Rabbit IgG polyclonal antibody for NACHT, LRR and PYD domains-containing protein 3 (NLRP3) detection. Tested with WB, IHC-P in Human, Mouse, Rat.

Synonyms:
AGTAVPRL antibody, AII/AVP antibody, Angiotensin/vasopressin receptor AII/AVP like antibody, Angiotensin/vasopressin receptor AII/AVP-like antibody, C1orf7 antibody, Caterpiller protein 1.1 antibody, CIAS 1 antibody, CIAS1 antibody, CLR1.1 antibody, Cold autoinflammatory syndrome 1 antibody, Cold autoinflammatory syndrome 1 protein antibody, Cryopyrin antibody, Familial cold autoinflammatory syndrome antibody, FCAS antibody, FCU antibody, LRR and PYD domains-containing protein 3 antibody, Muckle-Wells syndrome antibody, MWS antibody, NACHT antibody, NACHT LRR and PYD containing protein 3 antibody, NALP 3 antibody, NALP3 antibody, NALP3_HUMAN antibody, NLRP3 antibody, PYPAF 1 antibody, PYPAF1 antibody, PYRIN containing APAF1 like protein 1 antibody, PYRIN-containing APAF1-like protein 1 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the N-terminus of human CIAS1(12-31aa RYLEDLEDVDLKKFKMHLED), different from the related rat and mouse sequences by one amino acid.A synthetic peptide corresponding to a sequence at the N-terminus of human CIAS1(12-31aa RYLEDLEDVDLKKFKMHLED), different from the related rat and mouse sequences by one amino acid.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, rat .
Predicted to work with: mouse.

Applications: WB, IHC-P

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.