CISH polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
CISH(cytokine inducible SH2-containing protein),also called CIS, CIS-1, G18, SOCS, is an important negative regulator for inflammatory signaling and belongs to the suppressors of cytokine signaling(SOCS) family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. CISH controls interleukin-2 signaling, and variations of CISH with certain SNPs are associated with susceptibility to bacteremia, tuberculosis and malaria. The human CISH gene is mapped to chromosome 3p21.3 by FISH. The mouse gene is tightly linked to the Gnai2 gene on chromosome 9, a region syntenic to human chromosome 3p21. CIS expression was upregulated by lipopolysaccharide(LPS) or Cryptosporidium parvum exposure, and this upregulation involved downregulation of MIR98 and LET7, which relieved MIR98- and LET7-mediated translational repression of CIS. Gain- and loss-of-function studies showed that CIS accelerated degradation of IKBA and enhanced NFKB activation in cholangiocytes in response to LPS stimulation or C parvum exposure.

Description:
Rabbit IgG polyclonal antibody for Cytokine-inducible SH2-containing protein (CISH) detection. Tested with WB, IHC-P, ICC in Human, Mouse, Rat.

Synonyms:
BACTS2 antibody, cis 1 antibody, CIS antibody, CIS-1 antibody, CIS1 antibody, CISH antibody, CISH_HUMAN antibody, Cytokine inducible inhibitor of signaling type 1B antibody, Cytokine inducible SH2 containing protein antibody, Cytokine-inducible SH2-containing protein antibody, G18 antibody, Protein G18 antibody, SOCS antibody, Suppressor of cytokine signaling antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the C-terminus of human CISH(241-258aa LPLPRRMADYLRQYPFQL), identical to the related rat and mouse sequences.A synthetic peptide corresponding to a sequence at the C-terminus of human CISH(241-258aa LPLPRRMADYLRQYPFQL), identical to the related rat and mouse sequences.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, rat .
Predicted to work with: mouse.

Applications: WB, IHC-P, ICC

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.