CKMT1A/CKMT1B Polyclonal Antibody

Background:
Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins.

Synonyms:
Acidic-type mitochondrial creatine kinase, CKMT, CKMT1, CKMT1B, Creatine kinase mitochondrial 1 (ubiquitous), Creatine kinase mitochondrial 1B, Creatine kinase U type mitochondrial, Creatine kinase U-type, KCRU, Mia-CK, mitochondrial, U-MtCK, Ubiquitous mitochondrial creatine kinase, UMTCK

Immunogen: Synthetic peptide of human CKMT1A/CKMT1B

Swissprot: P12532
Gene Accession: NP066270

Calculated MW: 47 kDa
Observed MW: Refer to figures

Purification Method: Antigen affinity purification

Buffer: PBS with 0.05% NaN3 and 40% Glycerol, pH7.4

Concentration: 1.68 mg/mL
Dilution: WB 1:500-1:2000, IHC 1:40-1:200, ELISA 1:5000-1:10000

Storage:
Store at -20°C. Avoid freeze / thaw cycles.