ClassiX™ CleanUp

Reliable, Scalable, Cost-effective
ClassiX™ CleanUp is a magnetic bead–based solution for purification and size selection of double-stranded DNA following PCR amplification and next-generation sequencing (NGS) library preparation.
Part of the incoming ClassiX™ Toolbox, ClassiX™ CleanUp integrates seamlessly into standard SPRI (Solid Phase Reversible Immobilization) workflows.
Validated at ratios from 0.5× to 1.8×, it delivers predictable DNA recovery and size selection for high-throughput applications without adding protocol complexity.

Performance at a glance
ClassiX™ CleanUp is engineered for predictable SPRI-based DNA clean-up across a broad range of fragment sizes, input amounts, and buffer conditions.

ClassiX™ CleanUp: Core Performance Specifications
Parameter ClassiX™ CleanUp
Chemistry SPRI-based magnetic bead DNA purification
Target nucleic acid Double-stranded DNA
Fragment size range ≥100 bp (≥200 bp optimal)
Size selection Ratio-dependent (SPRI-standard behavior)
Bead-to-sample ratio 0.5× – 1.8× (application-dependent)*
Input DNA amount 5 ng – 200 ng
Input sample volume 10 – 50 µL
Elution volume 10 – 50 µL
Wash buffers 70% or 80% ethanol
Elution buffers Water, 10 mM Tris-Acetate pH 8.0, 10 mM Tris / 1 mM EDTA pH 8.0
Time to result ≤15 minutes
Workflow Bind – wash – elute
Automation compatibility Manual and semi-automated magnetic workflows
Intended use Research Use Only (RUO)

Why DNA clean-up matters
DNA purification is a critical control point in PCR and NGS workflows.
Inconsistent or suboptimal clean-up can result in:
- Variable DNA recovery
- Incomplete removal of primers, adapters, and enzymes
- Reduced library quality and complexity
- Increased variability between runs and operators

Reliable clean-up depends not only on chemistry, but on predictable size selection and recovery across bead ratios, input amounts, and buffer conditions. ClassiX™ CleanUp is designed to support reproducible downstream performance in both routine and high-throughput laboratory environments.

Size selection and bead ratio guidance
ClassiX Clean-up follows canonical SPRI size-selection behavior, enabling users to tune fragment retention by adjusting the bead-to-sample ratio.

Portfolio
ClassiX™ CleanUp (RUO) products are manufactured under controlled processes and verified for compatibility with standard SPRI-based purification workflows.

Automated DNA clean-up on the SwiftXtractor™ SL
ClassiX™ CleanUp is fully supported on the SwiftXtractor™ SL, automating the complete bind–wash–elute SPRI workflow for PCR product and NGS library purification. The walk-away protocol removes hands-on pipetting and ethanol wash handling, the steps where recovery variability between operators typically originates.
The automated protocol has been validated against the manual reference procedure, demonstrating equivalent DNA recovery and size-selection behaviour across bead-to-sample ratios, together with high run-to-run reproducibility. Validation data and the automated protocol are available on request.

Validated performance highlights
- FAST AND SIMPLE CLEAN-UP: Bind–wash–elute workflow completed in ≤ 15 minutes using standard laboratory equipment
- REPRODUCIBLE DNA RECOVERY: Consistent performance across runs and operators supports reliable downstream analysis
- WORKFLOW COMPATIBILITY: Compatible with standard magnetic racks, ethanol wash steps (70–80%), and commonly used elution buffers
- NGS-READY PERFORMANCE: Suitable for PCR product purification and NGS library clean-up, including adapter and primer removal

Workflow compatibility
ClassiX™ CleanUp is designed for straightforward integration into existing laboratory protocols:
- No changes to standard SPRI-based procedures
- Compatible with manual and semi-automated workflows
- Suitable for low- and high-throughput applications

Validated working ranges:
- Input volumes: 10–50 µL
- Elution volumes: 10–50 µL