Cleaved-Notch 1 (V1754) polyclonal, anti-human, mouse, rat

Cleaved-Notch 1 (V1754) polyclonal, anti-human, mouse, rat

€328.00
In stock
SKU
BS7018
Catalog Number: BS7018
Size: 50 ul, 100 ul
Isotype: rabbit IgG
Applications: IHC

Datasheet
Background:
Notch1 is a transmembrane protein functioning in development and the determination of cell fate. During maturation, the notch molecule is cleaved by a furin-like convertase at its extracellular domain. Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the carboxy-terminal notch1 fragment is released and further cleaved between Gly1753 and Val1754. The resulting activated cytosolic fragment translocates to the nucleus where it activates transcription.

Alternative Name:
Neurogenic locus notch homolog protein 1, Notch 1, hN1, Translocation-associated notch protein TAN-1, Notch 1 intracellular domain, NICD, NOTCH1, TAN1

Application Dilution: IHC: 1:50~1:200

Specificity: Cleaved-Notch 1 (V1754) polyclonal antibody detects endogenous levels of the Notch1 intracellular domain (NICD) . The antibody does not recognize full-length Notch1.

Immunogen:
Synthetic peptide, corresponding to amino acids 1700-1800 of Human Notch 1.

MW: ~ 110 kDa

Swis Prot.: P46531

Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Format:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.
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