Cleaved PARP (clone 7D10), anti-human

Background:
Poly [ADP-ribose] polymerase 1 (PARP-1) also known as NAD+ ADP-ribosyltransferase 1 or poly[ADP-ribose] synthase 1 is an enzyme that in humans is encoded by the PARP1 gene. PARP1 has a role in repair of single-stranded DNA (ssDNA) breaks. Knocking down intracellular PARP1 levels with siRNA or inhibiting PARP1 activity with small molecules reduces repair of ssDNA breaks. In the absence of PARP1, when these breaks are encountered during DNA replication, the replication fork stalls, and double-strand DNA (dsDNA) breaks accumulate.

Synonyms: PARP-1, Poly(ADP ribose) polymerase 1, sPARP1, ADPRT1, ADP ribosyltransferase NAD(+)

Cellular Location: Nucleus

Immunogen:
A synthetic peptide coupled to KLH

Gene Symbol: Cleaved PARP

Gene ID: 142

Swiss prot: P09874

Calculated MW: 116kDa(Pro)89kDa(Active)

Recommended dilution:
WB 1:1000-3000

Purity:
Affinity purification

Storage Buffer:
Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.

Storage:
Store at -20℃. Avoid freeze / thaw cycles.