Cleaved PARP Mouse mAb

Background:
Poly [ADP-ribose] polymerase 1 (PARP-1) also known as NAD+ ADP-ribosyltransferase 1 or poly[ADP-ribose] synthase 1 is an enzyme that in humans is encoded by the PARP1 gene. PARP1 has a role in repair of single-stranded DNA (ssDNA) breaks. Knocking down intracellular PARP1 levels with siRNA or inhibiting PARP1 activity with small molecules reduces repair of ssDNA breaks. In the absence of PARP1, when these breaks are encountered during DNA replication, the replication fork stalls, and double-strand DNA (dsDNA) breaks accumulate.

Specificity: Human

Alternative Names:
PARP-1, Poly(ADP ribose) polymerase 1, sPARP1,ADPRT1, ADP ribosyltransferase NAD(+)

Immunogen: Synthetic Peptide

Isotype: Mouse Monoclonal IgG1

Concentration: 1mg/ml

Recommended dilutions: WB 1:1,000-3,000, IHC 1:200-500

Observed band: 116,89kDa

GeneID: 142

Swiss-Prot No.: P09874

Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Store at -20°C. Avoid repeated freeze-thaw cycles.

NOTE: For Research Use Only