Cleaved PARP PARP1 monoclonal, anti-human

Description: Anti-Cleaved PARP PARP1 Rabbit Monoclonal Antibody . Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.

Protein Function:
Involved in the base excision repair (BER) pathway, by catalyzing the poly (ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly (ADP- ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites..

Other Names: Poly [ADP-ribose] polymerase 1, PARP-1, 2.4.2.30, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, DNA ADP-ribosyltransferase PARP1, 2.4.2.-, NAD(+) ADP-ribosyltransferase 1 {ECO:0000303|Ref.11}, ADPRT 1 {ECO:0000303|Ref.11}, Poly[ADP-ribose] synthase 1, Protein poly-ADP-ribosyltransferase PARP1, 2.4.2.-, Poly [ADP-ribose] polymerase 1, processed C-terminus, Poly [ADP-ribose] polymerase 1, 89-kDa form, Poly [ADP-ribose] polymerase 1, processed N-terminus, NT-PARP-1, Poly [ADP-ribose] polymerase 1, 24-kDa form, Poly [ADP-ribose] polymerase 1, 28-kDa form, PARP1 {ECO:0000303|PubMed:21680843, ECO:0000312|HGNC:HGNC:270}

Clone Name(s):
Clone: BHH-16

Subcellular Localization: Nucleus. Nucleus, nucleolus. Localizes at sites of DNA damage.

Gene ID: 142

Immunogen: A synthesized peptide derived from human Cleaved PARP

Calculated MW: 113084

Purification: Affinity-chromatography

Format: Liquid

Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Concentration (mg/ml): Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.