Cleaved-PARP1 (D214) Polyclonal Antibody

Background:
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.

Research Area: Cancer, Cell Biology, Epigenetics and Nuclear Signaling, Metabolism

Synonyms:
ADP-ribosyltransferase diphtheria toxin-like 1, ADPRT 1, ADPRT, ADPRT1, APOPAIN, ARTD1, NAD(+) ADP-ribosyltransferase 1, PARP, PARP-1, PARP1, PARP1, Poly [ADP-ribose] polymerase 1, Poly ADP ribose polymerase 1, Poly[ADP-ribose] synthase 1, PPOL, SCA1

Immunogen: Synthesized peptide derived from the Internal region of human PARP-1

Swissprot: P09874

Calculated MW: 113 kDa
Observed MW: 24 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4

Concentration: 1 mg/mL
Dilution: WB 1:500-2000, IHC 1:50-300, IF 1:50-300

Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Citation(s):
https://pubmed.ncbi.nlm.nih.gov/32862153/