Collagen I polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
Collagen, type I, alpha 1, also known as COL1A1, is a human gene that encodes the major component of type I collagen, the fibrillar collagen found in most connective tissues, including cartilage. This gene is mapped to 17q21.33. This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis.

Description:
Rabbit IgG polyclonal antibody for Collagen alpha-1 (I) chain (COL1A1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human, Mouse, Rat.

Synonyms:
Alpha 1 type I collagen antibody, Alpha 2 type I collagen antibody, Alpha-1 type I collagen antibody, CO1A1_HUMAN antibody, COL1A1 antibody, COL1A2 antibody, Collagen alpha-1(I) chain antibody, Collagen I alpha 1 polypeptide antibody, Collagen I alpha 2 polypeptide antibody, Collagen Of Skin Tendon And Bone antibody, Collagen Type 1 antibody, Collagen type I alpha 1 antibody, Collagen type I alpha 2 antibody, OI4 antibody, Osteogenesis Imperfecta Type IV antibody, Pro alpha 1(I) collagen antibody, Type I procollagen antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the C-terminus of mouse Collagen I(1192-1207aa QPPQEKSQDGGRYYRA), identical to the related rat sequence, and different from the related human sequence by two amino acids.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, mouse, rat

Applications: WB, IHC-P, IHC-F, ICC

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.