Collagen II polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
Collagen, type II, alpha 1, also known as COL2A1, is a human gene which is the major collagen synthesized by chondrocvtes. COL2A1 is mapped to 12q13.11. This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia.

Description:
Rabbit IgG polyclonal antibody for Collagen alpha-1 (II) chain (COL2A1) detection. Tested with WB in Human, Mouse, Rat.

Synonyms:
Alpha 1 type II collagen antibody, Alpha-1 type II collagen antibody, AOM antibody, Cartilage collagen antibody, Chondrocalcin antibody, CO2A1_HUMAN antibody, COL11A3 antibody, COL2A1 antibody, COL2A1 protein antibody, Collagen alpha 1(II) chain precursor antibody, Collagen II alpha 1 polypeptide antibody, Collagen type II alpha 1(primary osteoarthritis spondyloepiphyseal dysplasia congenital) antibody, MGC131516 antibody, SEDC antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the C-terminus of human Collagen II(1222-1241aa AFAGLGPREKGPDPLQYMRA), different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human.
Predicted to work with: mouse, rat.

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.