Collagen Type III polyclonal, anti-most species including human, rat, mouse, rabbit

Quantity: 50 µg

Background:
This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.

Synonyms:
COL3A1, EDS4A

Isotype: Rabbit Polyclonal

Immunogen:
Human placental type III collagen

Form: Purified IgG

Specificity:
This antibody is reactive with most collagen type III. Cross-reactivity of less than 1% with other collagens was detected by ELISA. Non-specific cross-reaction with other human serum proteins or non-collagen extracellular matrix proteins is negligible. Reactivity will be diminished with denatured collagen or formalin-fixed, paraffin-embedded tissues. It works best on non-denatured tissues.

Reactivity: most species including human, rat, mouse, rabbit

Applications: ELISA, Immunoblotting, Immunohistochemistry, suitable for frozen and paraffin sections (proteolytic treatment required; enzyme: pepsin). Suitable for use in immunostaining of extra or intracellular components in light microscopy

Working DIlution: ELISA: 1?g/well; Immunoblotting (non-denaturing): starting dilution: 1:5000; Immunohistochemistry (frozen): starting dilution: 1:50

Storage: 2-8C for immediate use, or at -20C (aliquot)