Complement C3d (clone BGRL 11) Affinity Purified, anti-human

Decription:
Mouse monoclonal antibody to human Complement C3d Affinity purified. The most abundant complement component is C3, which has molecular weight of 195kDa and is present in plasma at a concentration of around .2mg/ml. C3 is cleaved by C3 convertase into fragments including C3d. When erythrocytes are treated with a complement binding antibody such as anti-Lea both C3c, C4 and C3d can be demonstrated on the cell surface, but on the cells of patients with autoimmune haemolytic anaemia of the cold type, only C3d can be demonstrated. It is therefore important that an antiglobulin reagent should contain the appropriate component. However, small amounts of C3d and C4 are to be found on normal red cells so that the anti-C3d component in a general antiglobulin reagent must be at an adequate concentration for detecting C3d without giving a false positive with normal cells.

Synonyms: Complement C3d, C3d

Clone: BGRL 11

Isotype: Mouse IgM kappa light chain

Immunogen: The antigen was made in response to intact human erythrocytes coated with C3/C4

Reactivity: human

Form: Affinity purified, in 0.1 M glycine buffer, 1 M Tris buffer, pH 7.4, sterile filtered

Applications: Immunofluorescence, Flow Cytometry

Working Dilution: Optimal dilution should be performed by user

Storage: 2-8C for immediate use, or at -20C (aliquot)

References:
1. Dobbie D. Brazier D.M. Gardner B. Holburn A.M. (1987) Transfusion 27, 453-459.
2. Fruitstone M.J. (1978) Transfusion 18, 125.
3. Freedman J., Mollison P.L. (1976) Vox Sang 31, 241-57.
4. Mushens R.E. and Bakacs T. (1992) Transfusion 32, 430-434