Connexin 43 polyclonal, anti-human, mouse

Background:
The connexins are a group of gap junction proteins which form a hexamer to compose a connexon. Clusters of connexons form a gap junction through which low molecular weight proteins may diffuse from cell to cell. Several mammalian cells with malignant phenotypes exhibit decreased connexin expression and gap junction communication. In Src transformed cells, there is a decrease in gap junctional communication, which appears to be associated with tyrosine phosphorylation of connexin 43. Activated c-Src phosphorylates the C-terminal tail of connexin 43 on Tyr 265, resulting in a stable interaction between both proteins, which leads to inhibition of gap junctional communication. In addition to tyrosine phosphorylation, connexin 43 has also been shown to be phosphorylated on serine in the absence of Src kinases and on both serine and tyrosine in cells expressing Src kinases, such as c-Src and/or pp60v-Src. In human vascular endothelial cells, connexin 43 is posttranslationally modified during mitosis. Mitosis-specific phosphorylation of connexin 43 correlates with the transient loss of gap junction intercellular communication and redistribution of connexin 43.

Alternative Name:
Cx43, GJA1, GJAL, Connexin 43, Connexin-43, Cx 43, Cx43, CXA1, DFNB38, Gap junction 43 kDa heart protein, Gap junction alpha-1 protein, Gap junction protein alpha 1 43kDa (connexin 43), Gap junction protein alpha 1 43kDa, Gap junction protein alpha like, GJA 1, Gja1, GJAL, ODD, ODDD, ODOD, SDTY3,

Application Dilution:
WB:1:500-1:2,000
IHC:1:50-1:200
FC:1:50-1:200

Specificity: Connexin 43 polyclonal antibody detects endogenous levels of Connexin 43 protein.

Immunogen:
Synthetic peptide within C-terminal human Connexin 43.

MW: 43 kDa

Swiss-Prot: P17302(Human) P23242(Mouse)

Purification & Purity:
Peptide affinity purified.

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.