Cortactin (phospho-Y466) polyclonal, anti-human, mouse, rat
€328.00
In stock
SKU
BS4779
Background:
Cortactin is an 80/85 kDa cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino terminal tandem repeats of a unique 37 amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an alpha helical structure followed by a proline rich region. Between the proline rich region and the SH3 domain, there are a series of Src phosphorylation sites (Tyr421, Tyr470, and Tyr486). In vitro, cortactin binds to and cross links F actin into meshworks.
Alternative Name:
Src substrate cortactin, Amplaxin, Oncogene EMS1, CTTN, EMS1
Application Dilution: WB: 1:500~1:1000, IHC: 1:50~1:200
Specificity: p-Cortactin (Y466) polyclonal antibody detects endogenous levels of Cortactin protein only when phosphorylated at Tyr466.
Immunogen:
Synthetic peptide, corresponding to amino acids 360-418 of Human TFDP1.
MW: ~ 85 kDa
Swis Prot.: Q14247
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
Cortactin is an 80/85 kDa cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino terminal tandem repeats of a unique 37 amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an alpha helical structure followed by a proline rich region. Between the proline rich region and the SH3 domain, there are a series of Src phosphorylation sites (Tyr421, Tyr470, and Tyr486). In vitro, cortactin binds to and cross links F actin into meshworks.
Alternative Name:
Src substrate cortactin, Amplaxin, Oncogene EMS1, CTTN, EMS1
Application Dilution: WB: 1:500~1:1000, IHC: 1:50~1:200
Specificity: p-Cortactin (Y466) polyclonal antibody detects endogenous levels of Cortactin protein only when phosphorylated at Tyr466.
Immunogen:
Synthetic peptide, corresponding to amino acids 360-418 of Human TFDP1.
MW: ~ 85 kDa
Swis Prot.: Q14247
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
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