Cotinine (Cot) test kit

Use: Used to detect the concentration of cotinine (Cot) in samples such as serum, plasma, and cell culture supernatant.Specificity: The kit uses enzyme-linked immunosorbent assay. Biotin-labeled cotinine (Cot) is used, and purified anti-cotinine (Cot) antibodies are coated on microplates. In the competitive inhibition reaction, a certain amount of solid-phase antibodies inhibit the competitive reaction with biotin-labeled cotinine (Cot) and non-labeled antigens (calibrators or specimens). The binding amount of the antibody and biotin-labeled cotinine (Cot) is inhibited by the amount of non-labeled antigen. The more non-labeled antigen, the less binding of the antibody and biotin-labeled cotinine (Cot), and vice versa. After the reaction is balanced, a solid-phase antibody-biotinylated cotinine (Cot) is formed, and then enzyme-labeled avidin is added to form a solid-phase antibody-biotinylated cotinine (Cot)-enzyme label-avidin complex. After adding substrate for color development, the absorbance (OD value) is measured at a wavelength of 450nm using an enzyme reader. As the concentration of cotinine (Cot) increases, the OD value gradually decreases, showing a good linear relationship. This kit has the characteristics of high sensitivity, strong specificity, good repeatability, simple operation and rapidity, and has reliable detection performance for the reduction or increase of cotinine (Cot) in samples.

Storage Conditions
Store at 2-8°C.

Use: The kit is for research use only.