Cpn10 polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
HSPE1(heat shock 10kDa protein 1(chaperonin 10)), also called CPN10, GROES, CHAPERONIN 10 HOMOLOG, cpn10 HOMOLOG or HSP10, is a protein that in humans is encoded by the HSPE1 gene. GroES is a heptameric ring of identical 10.4-kD subunits that binds to each end of GroEL to form a symmetric, functional heterodimer. The HSP10 gene consists of 4 exons. The HSPE1 gene is mapped to 2q33.1. The transcriptional activity of the promoter fragment in the HSP60 direction is approximately twice that in the HSP10 direction under normal growth conditions; upon heat shock, promoter activity in either direction increased by a factor of approximately 12. Mutational drifts performed in vitro with 4 different enzymes indicated the GroES overexpression doubled the number of accumulating mutations, and promoted the folding of enzyme variants carrying mutations in the protein core and/or mutations with higher destabilizing effects.

Description:
Rabbit IgG polyclonal antibody for 10 kDa heat shock protein, mitochondrial (HSPE1) detection. Tested with WB, IHC-P, ICC in Human, Mouse, Rat.

Synonyms:
10 kDa chaperonin antibody, 10 kDa heat shock protein mitochondrial antibody, 10 kDa heat shock protein, mitochondrial antibody, CH10_HUMAN antibody, Chaperonin 10 antibody, Cpn 10 antibody, CPN10 antibody, Early pregnancy factor antibody, Early-pregnancy factor antibody, EPF antibody, GROES antibody, Heat shock 10kD protein 1 antibody, Heat shock 10kD protein 1 chaperonin 10 antibody, Heat shock 10kDa protein 1 antibody, Heat shock 10kDa protein 1 chaperonin 10 antibody, HSP 10 antibody, HSP10 antibody, HSPE 1 antibody, HSPE1 antibody.

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the C-terminus of human Cpn10(81-102aa VVLDDKDYFLFRDGDILGKYVD), different from the related rat and mouse sequences by one amino acid.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, mouse, rat

Applications: WB, IHC-P, ICC

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.