CPT2 polyclonal, anti-human, mouse, rat

Background:
The mitochondrial β-oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase (CPT) I (outer membrane and detergent labile) and II (inner membrane and detergent stable), together with carnitine carrier. CPTI catalyzes the first reaction in the transport of long-chain fatty acids from the cytoplasm to the mitochondrion, a rate-limiting step in beta-oxidation. Two types of CPTI are known, the liver (CPTIA) and muscle (CPTIB) isoforms. The muscle type protein is specially expressed in heart and skeletal muscle. Membrane-bound CPTI, but not CPTII, is inhibited reversibly by malonyl-coenzyme A (CoA). Unlike CPTII, CPTI requires membrane integrity for catalytic function. In addition, glutamic acid 3 and histidine 5 are necessary for malonyl CoA inhibition and binding to liver CPTI, but not for catalytic activity.

Alternative Name:
CPT II, CPT2, Carnitine O palmitoyltransferase 2, Carnitine O palmitoyltransferase 2 mitochondrial, Carnitine O-palmitoyltransferase 2, Carnitine palmitoyltransferase 2, Carnitine palmitoyltransferase II, CPT 1, CPT 2, CPT II, CPT1, CPT2, CPT2, CPTASE, CPTII, IIAE4, mitochondrial,

Application Dilution:
WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200

Specificity: CPT2 polyclonal antibody detects endogenous levels of CPT2 protein.

Immunogen:
recombinant protein

MW: 74 kDa

Swiss-Prot: P23786(Human) P52825(Mouse) P18886(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.