CREBZF Polyclonal Antibody

Background:
Host cell factor (HCF) is a cellular cofactor required for the activation of VP16 which expresses the herpes simplex virus immediate early gene. V16 binds to HCF through a 4-amino acid motif similar to the HCF binding domain of the basic leucine-zipper proteins Luman and Zhangfei (ZF). Luman activates promoters containing cAMP or unfolded protein response elements (UPRE). Zhangfei suppresses the transcriptional activity of Luman, but requires HCF binding which may target Luman and Zhangfei to a common location. Sequence analysis predicts that the deduced 272-amino acid Zhangfei protein has a negatively charged N terminus, a leucine zipper of 6 heptad leucine repeats separated by a conserved 6-amino acid spacer, a basic domain, and a bZIP region. It is also presumed that the N-terminal acidic region of Zhangfei is an activation domain.

Research Area: Cancer, Epigenetics and Nuclear Signaling

Synonyms:
1110034C16Rik, 6330417B10Rik, AI225749, AI606244, CREB/ATF bZIP transcription factor, Crebzf, FLJ94018, HCF-binding transcription factor Zhangfei, Host cell factor-binding transcription factor Zhangfei, LAZip, LAZipII, SHP-interacting leucine zipper protein, SMILE, ZF, ZHANG

Immunogen: Synthetic peptide of human CREBZF

Swissprot: Q9NS37
Gene Accession: NP001034707

Calculated MW: 37 kDa
Observed MW: Refer to figures

Purification Method: Antigen affinity purification

Buffer: PBS with 0.05% NaN3 and 40% Glycerol, pH7.4

Concentration: 0.4 mg/mL
Dilution: WB 1:500-1:2000, IHC 1:25-1:100, ELISA 1:2000-1:5000

Storage:
Store at -20°C. Avoid freeze / thaw cycles.