CtBP1 polyclonal, anti-human, mouse, rat

Background:
CtBP1 is a cellular phosphoprotein that associates with various proteins and functions as a corepressor of transcription. CtBP1 and the related protein CtBP2 are characterized as C-terminal binding protein of adenovirus E1A, and they preferentially associate with the E1A via a 5-amino acid motif, PLDLS, to repress E1A induced oncogenesis and cellular transformation. CtBP1 is expressed from embryo to adult, but CtBP2 is mainly expressed during embryogenesis. During skeletal and T-cell development, CtBP1 and CtBP2 associate with the PLDLSL domain of δEF1, a cellular zinc finger-homeodomain protein, and thereby enhances δEF1 induced transcriptional silencing. In addition, CtBP complexes with CtIP, a protein that recognizes distinctly different protein motifs from CtBP. CtIP binds to the BRCT repeats within the breast cancer gene BRCA1 and enables CtBP to influence BRCA1 activity. CtIP/CtBP binding to BRCA1 inhibits the transactivation of the p21 promoter, and it is critical for regulating p21 transcription in response to DNA damage.

Alternative Name:
CtBP1, CTBP1, CTBP, BARS, brefeldin A- ribosylated substrate, C terminal binding protein 1, C-terminal-binding protein 1, CTBP, CtBP1, CTBP1, MGC104684,

Application Dilution:
WB:1:500-1:2,000
IHC:1:50-1:200
ICC:1:50-1:100
IP:1:10-1:50

Specificity: CtBP1 polyclonal antibody detects endogenous levels of CtBP1 protein.

Immunogen:
Recombinant protein within human CtBP1 aa 300-500.

MW: 47 kDa

Swiss-Prot: Q13363(Human) Q9Z2F5(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.