CTCF polyclonal, anti-human, mouse, rat

Background:
CTCF belongs to the zinc finger transcription factor family, and it recognizes unusually long and remarkably divergent DNA target sequences to influence expression of many various genes. The DNA-binding domain of CTCF is composed of 11 Zn fingers including 10 that are of C2H2-class, and 1 that is of C2HC class, and they are highly conserved between vertebrate species. CTCF functions as a repressor of the c-myc gene and as a regulator of lysozyme gene expression. In addition, CTCF associates with the essential activator domain in the promotor region of the amyloid β-protein precursor (APP) gene to activate transcription of APP. Expression of CTCF up-regulates APP expression and thereby, enhances synapse formations between primary neurons during development.

Alternative Name:
11 zinc finger protein, 11 zinc finger transcriptional repressor, 11-zinc finger protein, CCCTC binding factor (zinc finger protein), CCCTC binding factor, CCCTC-binding factor, Ctcf, CTCF, CTCFL paralog, Transcriptional repressor CTCF,

Application Dilution: WB: 1:1000 - 1:2000, IHC/IF: 1:50 - 1:200, IP/ChIP: 1:50 - 1:100

Specificity: CTCF polyclonal antibody detects endogenous levels of CTCF protein.

Immunogen:
Recombinant full length Human CTCF.

MW: ~ 83 kDa

Swis Prot.: P49711

Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Format:
1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol.

Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.