CUEDC2 polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
CUEDC2(Cue Domain-Containing Protein 2) is involved in ubiquitin-and proteasome-mediated degradation of progesterone receptor(PR, or PGR) and estrogen receptor(ER)-alpha. Hartz(2011) mapped the CUEDC2 gene to chromosome 10q24.32 based on an alignment of the CUEDC2 sequence with the genomic sequence(GRCh37). Using coimmunoprecipitation analysis and protein pull-down assays, Zhang et al.(2007) confirmed that CUEDC2 interacted with the PRB isoform of PR. Mutation analysis revealed that the IF domain of PRB and the CUE domain of CUEDC2 were required for the interaction. CUEDC2 reduced PRB protein content and promoted progesterone-induced PRB degradation via the ubiquitin-proteasome pathway.

Description:
Rabbit IgG polyclonal antibody for CUE domain-containing protein 2 (CUEDC2) detection. Tested with WB, IHC-P in Human, Mouse, Rat.

Synonyms:
bA18I14.5 antibody, C10orf66 antibody, Chromosome 10 open reading frame 66 antibody, CUE domain containing 2 antibody, CUE domain-containing protein 2 antibody, CUED2 antibody, CUED2_HUMAN antibody, Cuedc2 antibody, MGC2491 antibody, OTTHUMP00000020368 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the C-terminus of human CUEDC2(267-287aa EAEEMKATYINLKPARKYRFH), identical to the related mouse sequence, and different from the related rat sequence by one amino acid.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human.
Predicted to work with: mouse, rat.

Applications: WB, IHC-P

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.