Cullin 1 polyclonal, anti-human, mouse, rat

Background:
Cullin proteins comprise a distinct family of mediators that participate in the selective targeting of proteins for ubiquitin (Ub)-mediated proteolysis. CUL-1, which is the mammalian homolog of yeast Cdc53, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF (Skp1/CUL-1/F-box protein complex) consists of Skp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. CUL-1-mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3, facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase. Proteolysis also occurs by way of CUL-4 associating with Nedd-8, a ubiquitin-like protein, where it too functions as an active component of a multifunctional E3 complex. CUL-5, also designated vasopressin-activated, calcium-mobilizing protein (VACM-1), is also included in the cullin family as it shares substantial sequence homology with CUL-1.

Alternative Name:
CUL-1, CUL1, CUL 1, CUL-1, CUL1, CUL1, Cullin-1, Cullin1, MGC149834, MGC149835,

Application Dilution:
WB:1:500-1:2,000
IHC:1:50-1:200
FC:1:50-1:100
IP:1:10-1:50
ICC/IF:1:10-1:50

Specificity: Cullin 1 polyclonal antibody detects endogenous levels of Cullin 1 protein.

Immunogen:
Recombinant protein

Swiss-Prot: Q13616(Human) Q9WTX6(Mouse)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.