Cullin 2 polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
Cullin 2(CUL2) is a protein that in humans is encoded by the CUL2 gene. Using immunofluorescence, they showed that CUL2 is a cytosolic protein that can be translocated to the nucleus by VHL. By fluorescence in situ hybridization, Clifford et al.(1999)mapped the CUL2 gene to 10p11.2-p11.1, a region reported to show loss of heterozygosity(LOH) in several forms of human cancer, including non-clear cell renal cell carcinoma. Pause et al.(1997)suggested that CUL2 is a candidate tumor suppressor gene, as has been proposed for CUL1(603134). Lonergan et al.(1998) demonstrated that formation of the VBC-CUL2 complexes is linked to the regulation of hypoxia-inducible mRNAs by VHL. Cul2 was one of several proteins required for degradation of a class of RNA-binding germline proteins in somatic cells of the early blastomere.

Description:
Rabbit IgG polyclonal antibody for Cullin-2 (CUL2) detection. Tested with WB in Human, Mouse, Rat.

Synonyms:
Cul2 antibody, CUL 2 antibody, CUL-2 antibody, CUL2 antibody, CUL2_HUMAN antibody, cullin 2 antibody, cullin homolog 2 antibody, Cullin-2 antibody, MGC131970 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence in the middle region of human Cullin 2(264-280aa HECQQRMVADHLQFLHA), different from the related mouse and rat sequences by one amino acid.A synthetic peptide corresponding to a sequence in the middle region of human Cullin 2(264-280aa HECQQRMVADHLQFLHA), different from the related mouse and rat sequences by one amino acid.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, rat .
Predicted to work with: mouse.

Applications: WB

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.